The second cohort of the Ready2Scale Acceleration Program is empowering a new wave of deep-tech startups across Europe with tailored mentorship, valuable resources, and €60,000 grants. One of the standout participants is BioHalo ApS, a Danish startup bringing sustainability to the chemical industry through bio-based innovation.
BioHalo is transforming the traditional halogenated chemical industry with an environmentally friendly approach. At the core of their work is the development of microorganisms that can produce high-performance monofluorinated fine chemicals. This innovative biofluorination technology offers a sustainable alternative to conventional fluorochemistry—particularly relevant in replacing harmful per- and polyfluoroalkyl substances (PFAS), also known as “forever chemicals,” without compromising on performance or quality.
The startup was co-founded by three scientists and entrepreneurs: Dr. Nicolas Krink (CEO), a synthetic biology expert with experience in venture capital; Mariela Mezzina; and Pablo Nikel—both renowned biopolymer experts and metabolic engineers. Together, their expertise spans synthetic biology, metabolic engineering, and strategic commercialization.
The following questions are answered by Dr. Nicolas Krink, CEO of BioHalo ApS.
How did the idea for your startup originate?
The idea behind BioHalo came from a deep understanding of the serious environmental and health risks posed by PFAS, or “forever chemicals.” We knew there had to be a better way to tackle this challenge. During our research at DTU Biosustain, we saw an opportunity not just in the science, but in how we could make a real-world impact. That’s what inspired us to start BioHalo ApS and turn our research into something that could make a tangible difference.
What have been your biggest challenges and most significant achievements so far?
One of our biggest challenges early on was figuring out how to produce sufficient amounts of our fine chemicals. At the beginning, we had an unoptimized method, and it is difficult to move forward when you do not have enough product to really understand its properties or do any prototyping with partners. Scaling is something that almost every university spinout faces, and it is definitely a key hurdle. However, we are very proud of the progress we have made so far. Joining the BioInnovation Institute incubator in Denmark was a significant milestone—it provided financial support, expert mentoring, and guidance we needed to accelerate our technology. In addition, participating in programs like EY’s Amplifier, AdMaCom by INAM, the Nucleate Activator, Plug and Play Japan, and Ready2Scale have all been important steps forward for us.
What drives you to continue, even during difficult times?
For me personally, I often feel that if something is really tough, it usually means it actually matters. The challenges we face are a reminder that we are working on something big, trying to drive real change by creating sustainable alternatives to PFAS without sacrificing performance. That sense of purpose is very motivating. It keeps me going, especially during the harder moments. I also believe we have brought together a strong and committed team that is ready to take on these complex challenges, which gives me a lot of confidence in what lies ahead.
What sets your startup apart from competitors?
What really sets us apart are two key things. First, our technology. I genuinely think we have developed something very unique and scientifically exciting with our biofluorination platform. It allows us to create sustainable, eco-friendly alternatives to PFAS that still deliver the high performance the industry expects—something most other solutions just cannot replicate. And second, our team. We have a strong, reliable group of people who are not only deeply knowledgeable but also truly committed to making this work. It’s this combination of breakthrough science and a capable team that gives us a real edge in the field.
What are your expectations from the Ready2Scale Acceleration Programme, and how do you think it will benefit your startup?
We see the Ready2Scale Acceleration Programme as a key part of our scale-up journey. It gives us access to expert advice, strong industry connections, and focused support, which really helps us navigate both technical and commercial challenges. From our experience with similar programmes like AdMaCom by INAM and the EY Amplifier, you get two main things. You are challenged—by the mentors, by the structure—it pushes you to stay sharp and make progress. And at the same time, you are inspired. Being around other driven startups and experienced mentors creates a strong energy. That combination of challenge and inspiration is what makes these programmes so valuable.
Where do you see your startup in one year? And in five years?
At BioHalo, we are pretty ambitious, so in one year we see ourselves having upscaled production to the point where we can really start working closely with customers on launching something new. Looking ahead five years, our goal is to be a global leader in sustainable, high-performance alternatives to PFAS—actually replacing some of the fossil-based fine chemicals out there with greener, bio-based solutions using our biofluorination technology.
Which books, podcasts, publications, or influencers do you follow and recommend to other entrepreneurs?
Being a founder means my time is spread across a lot of responsibilities, so I rarely have the luxury to sit down with books, podcasts, or long interviews. That is why I rely on tools like ChatGPT and Blinkist—they give me quick, efficient summaries that help me stay sharp and keep learning without slowing down my day.
BioHalo is not actively hiring at the moment, but the team is always open to connecting with talented individuals—especially those with a background in fermentation, organic chemistry, polymers, or application development. They also welcome interest from master’s students looking to complete their thesis projects in a dynamic, mission-driven environment. If BioHalo’s vision resonates with you and you’d like to explore potential opportunities, don’t hesitate to reach out via their website or drop a message on LinkedIn.